Development of genetic tools
We are interested in developing novel genetic tools that can facilitate neuroscience research in fruit flies Drosophila and malaria mosquitoes Anopheles gambiae. We developed the second generation of Q-system in Drosophila and introduced the Q-system into A. gambiae by genetically labelling olfactory receptor neurons of the mosquitoes.
Our latest project has introduced split-QF into Drosophila and validated its use in a number of proof-of-principle experiments. We also confirmed that split-QF works with split-GAL4 and split-LexA, thus greatly expanding the possible applications of all three systems.
Read more here:
Riabinina O, Vernon SW, Dickson BJ, Baines RA. (2019) Split-QF system for fine-tuned transgene expression in Drosophila. Genetics, 212, 1, 53-63. link
Riabinina O, Task D, Marr E, Lin C-C, Alford R, O’Brochta DA, Potter CJ. (2016) Organisation of olfactory centers in the malaria mosquito Anopheles gambiae. Nature Communications, 7, 13010. link
Riabinina O, Potter CJ. The Q-system: A versatile Expression System for Drosophila. In Drosophila: Methods and Protocols (ed: C. Dahmann) (Methods in Molecular Biology, Vol. 1478, 53-78) link
Riabinina O, Luginbuhl D, Marr E, Liu S, Wu MN, Luo L, Potter CJ. (2015) Improved and expanded Q-system reagents for genetic manipulations. Nature Methods, 12, 219-222 link